Journal article
Characterization of CTX-M-15-Klebsiella pneumoniae from inpatients and outpatients of a teaching hospital


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Publication Details
Author list: Muzaheed Muzaheed , Saeed Sattar Shaikh , Sadananda Acharya, Shajiya Sarwar Moosa, Mohammad Habeeb Shaikh , Faisal M. Alzahrani, Amer Ibrahim Alomar
Publisher: F1000Research / F1000 Research Ltd
Publication year: 2021
Journal: F1000Research
Volume number: 10:444
Issue number: 10:444
Start page: 1
End page: 9
Number of pages: 9
ISSN: 2046-1402
Web of Science ID:
PubMed ID:
Scopus ID:
eISSN: 2046-1402


Background 
The presence of Extended-spectrum β-lactamase (ESBL) positive bacteria in hospital setting is an aggravating influential factor for hospitalized patients, and its consequences may be hazardous. Therefore, there is a need for rapid detection methods for newly emerging drug-resistant bacteria. This study was aimed at the molecular characterization of ESBL-positive Klebsiella pneumoniae isolates recovered from clinical samples.  
Methods 
A total of 513 K. pneumoniae isolates were obtained from various clinical samples during June 2019 to May 2020. The collected isolates were investigated for antimicrobial susceptibility (antibiogram), and PCR and DNA sequencing were performed to analyse the ESBL genes.  
Results 
Among the 513 isolates, as many as 359 (69.9%) were ESBL producers and 87.5% were multi-drug resistant, while none had resistance to imipenem. PCR scored 3% blaTEM, 3% blaSHV, and 60% blaCTX-M-15 genes for the tested isolates.  
Conclusion 
The study showed that CTX-M-15 was the major prevalent ESBL type among the isolates. Additionally, all the isolates were susceptible to carbapenems. Screening and detection of ESBL tests are necessary among all isolates from the enterobacteriaceae family in routine microbiology laboratory to prevent associated nosocomial infections. A larger study is essential to understand molecular epidemiology of ESBL producing organisms to minimize morbidities due to these multidrug resistant organisms.


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Last updated on 2021-12-09 at 10:12