Journal article
mCIM test as a reliable assay for the detection of CRE in the Gulf region


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Author list: Safiya Almusawi, Jawad Ur Rahman, Salma Ali Aljaroodi, Lateefah Alshammari, Ahmed Itbaileh, Hessa Mohammed​, Nermin Saeed​, Baha Abdalhamid​, Khaled R. Alkharsah, and Reem Y. Aljindan
Publisher: Microbiology Society
Publication year: 2021
Journal: Journal of Medical Microbiology
Journal acronym: JMM
Volume number: 70
Issue number: 7
Start page: 1
End page: 10
Number of pages: 10
ISSN: 0022-2615
Web of Science ID: 000670510800001
PubMed ID: 34232118
Scopus ID: 85111249339
eISSN: 1473-5644


Introduction. Carbapenem resistant Enterobacterales (CRE) are one of the leading causes of systemic and nosocomial infections and are multidrug-resistant organisms producing different carbapenemases. There are many genotypic and phenotypic methods for detecting the carbapenemases; however, there is a limitation for each. Modified carbapenem inactivation method (mCIM) assay is a recent phenotypic method which has been published by the Clinical and Laboratory Standards Institute.

Hypothesis / Gap Statement. mCIM assay could provide a reliable method for the detection of carbapenemases in CRE.

Aim. Evaluation of the mCIM assay performance for the detection of carbapenemases in Enterobacterales and the identification of the common carbapenemase genes at Eastern Province of Saudi Arabia and Kingdom of Bahrain.

Methodology. A collection of 197 non-duplicate carbapenem resistant Enterobacterales clinical isolates, were evaluated with the mCIM test comparing its performance to multiplex PCR. The minimum inhibitory concentration susceptibility testing was done by the Etest method for imipenem, meropenem, and ertapenem.

Results. The sensitivity of the mCIM assay was 94 % (95 % CI, (89.3–97.1)). In Saudi Arabia and Bahrain, OXA-48 was the most prevalent carbapenemase gene followed by NDM. Coexistence of multiple carbapenemase genes is reported in eleven cases.

Conclusion. These findings indicate that the mCIM test is a reliable and simple assay for detecting the activity of carbapenemase in Enterobacterales , especially in resource-limited laboratories.


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Last updated on 2021-31-08 at 09:23