Journal article
The effect of safranal loaded mucoadhesive nanoemulsion on oxidative stress markers in cerebral ischemia

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Publication Details
Author list: Niyaz Ahmad, Rizwan Ahmad, Atta Abbas Naqvi, Mohammad Ashafaq, Md Aftab Alam, Farhan Jalees Ahmad & Mastour Safer Al-Ghamdi
Publisher: Informa Healthcare
Publication year: 2017
Journal: Artificial Cells, Nanomedicine and Biotechnology
Journal name in source: Artificial Cells, Nanomedicine and Biotechnology
Volume number: 45
Issue number: 4
Start page: 775
End page: 787
Number of pages: 13
ISSN: 2169-141X
Web of Science ID: 000399487000014
PubMed ID: 27609117
Scopus ID: 84986183103
eISSN: 2169-141X

Antioxidants, with reported neuroprotective activity, encounter free radical induced neural damage leading to reduced risk of cerebral ischemia–reperfusion (IR) injury. Safranal, an antioxidant drug with potential role in the amelioration of cerebral ischemia, endures low solubility and poor absorption property thus resulting a low serum and tissue bioavailability. This research aims to prepare nanoemulsion with the concept; to increase the bioavailability in order to reduce oxidative stress-induced brain injury as well as to evaluate the brain-drug targeting following non-invasive nasal route administration in middle cerebral artery occlusion (MCAO) animal model. Titration method was used to prepare safranal mucoadhesive nanoemulsion (SMNE) followed by further characterization, i.e. entrapment efficiency, particles size, and zeta potential study. Optimized SMNE showed; mean globule size of 89.64 nm (±9.12), zeta potential –11.39 mV (±1.32), drug content 98.47% (±1.01), and viscosity of 124 cp (±14). Rats were subjected to 2 h of MCAO, successively followed by a 22 h reperfusion, after which the grip strength, locomotor activity, and biochemical studies, i.e. glutathione reductase (GR), glutathione peroxidase, lipid peroxidation, catalase, and superoxide dismutase were studied as assessment tool for effective treatment in brain. SMNE administered i.n. (intranasal) in MCAO induced cerebral ischemia rats exhibited significant improvement in neurobehavioral (locomotor and grip strength) and antioxidant activity as well as histopathological studies. The toxicity studies performed at the end revealed safe nature of developed SMNE.

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Last updated on 2018-29-08 at 13:53